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Regulatory Tasks of the Phosphoenolpyruvate-Phosphotransferase System of Pseudomonas putida in Central Carbon Metabolism

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Источник: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3312210/
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Similar-sized protein separation

MIL-100(Cr) PE MMM-86% membrane was mounted a dead-end filtration cell. First, 3.3 mg of BSA (66 kDa) and 3.3 mg of BHb (65 kDa) were dissolved into the 10 mL of deionized water. Then, 1 mL of mixture was taken from initial solution and re-dispersed into 9 mL of buffer solution at pH = 4.7 (total concentration = 0.066 mg mL−1). The separation time was varied from 1 to 5 h with 1 h of the interval. Collected samples at 1–5 h were dried in 70 °C vacuum oven and re-dispersed in the excessive amount of buffer (2 mL).